There are many animals that are located in the Wekiva Basin including several designated as endangered, threatened, or of special concern.

The wood stork, an endangered species, nests in cypress trees within the aquatic preserve, and is often observed feeding in certain shallow areas of the river. The little blue heron, tri-colored heron and limpkin, species of special concern, nest and forage along the banks of the Wekiva.

Deer range from the high sandhills and scrub down through the flatwoods to the hammocks and swamps. Gray fox, bobcat, raccoon, opossum and black bear range across the entire park and are not restricted to any one habitat. Not only is "variety the spice of life", it is also the key to attracting and supporting a greater variety of life.